HIV Testing Algorithm

Step 1: HIV-1/2 Ag-Ab Screening Assay Step 2: HIV-1/2 Ab Differentiation Assay Step 3: HIV-1 RNA Assay Interpretation for Labratory Report
Non-Reactive N/A N/A Negative for HIV-1 antigen and HIV-1/HIV-2 antibodies. No laboratory evidence of HIV infection.
Reactive HIV-1 Positive N/A Positive for HIV-1 antibodies. Laboratory evidence of HIV-1 infection is present.
Reactive HIV-2 Positive N/A Positive for HIV-2 antibodies. Laboratory evidence of HIV-2 infection is present.
Reactive Non-Reactive or Any indeterminate results (HIV-1, HIV-2 or HIV) Detected Positive for HIV-1. Laboratory evidence consistent with acute or early HIV-1 infection is present.
Reactive Non-Reactive or Any indeterminate results (HIV-1, HIV-2 or HIV) Not detected HIV antibodies were not confirmed and HIV-1 RNA was not detected. Possible false positive. Further testing is recommended if warranted by clinical evaluation or risk factors.
Reactive HIV Positive Untypable N/A Positive for HIV antibodies. Laboratory evidence of HIV infection is present. Antibodies not differentiated as HIV-1 or HIV-2. HIV-1 RNA and HIV-2 RNA or DNA testing is recommended.